3LOT

Crystal structure of PROTEIN OF UNKNOWN FUNCTION (NP_070038.1) from ARCHAEOGLOBUS FULGIDUS at 1.89 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-14
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97936,0.97922
Spacegroup name	P 21 21 21
Unit cell lengths	75.786, 103.079, 156.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 1.890
R-factor	0.159
Rwork	0.156
R-free	0.20600
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.537
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.970	48.970	1.960
High resolution limit [Å]	1.890	4.060	1.890
Rmerge	0.107	0.040	0.552
Number of reflections	98016	10168	9838
<I/σ(I)>	9.84	26.6	2.2
Completeness [%]	99.2	97.3	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.2000M ammonium acetate, 30.0000% polyethylene glycol 4000, 0.1M sodium citrate pH 5.6, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 277K