3LOP
Crystal structure of substrate-binding periplasmic protein (Pbp) from Ralstonia solanacearum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.110, 64.739, 114.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.720 - 1.550 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.19500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.720 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.078 | 0.162 |
| Number of reflections | 46380 | |
| <I/σ(I)> | 22.4 | 2.5 |
| Completeness [%] | 97.2 | 95.3 |
| Redundancy | 14.4 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Potassium Thiocynate, 25% Polyethylene Glycol Monomethyl ether 2000, 0.1M Nickel (II) Chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
