3LOB
Crystal Structure of Flock House Virus calcium mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 477.088, 404.929, 476.189 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.600 |
| R-factor | 0.347 |
| Rwork | 0.347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FHV wildtype structure |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.490 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.103 | 0.290 |
| Number of reflections | 372968 | |
| <I/σ(I)> | 5.73 | 1.37 |
| Completeness [%] | 35.9 | 17.5 |
| Redundancy | 1.3 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 295 | 100 mM sodium citrate, pH 4.0, 4% PEG 8000 and 100 mM lithium sulfate, VAPOR DIFFUSION, temperature 295K |
