3LO8

Crystal Structure of The Oxidized Form of Ferredoxin:NADP+ Reductase From Maize Root at 1.05 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	59.140, 59.140, 186.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.050
R-factor	0.125
Rwork	0.125
R-free	0.15540
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1jb9
RMSD bond length	0.015
RMSD bond angle	0.208
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.070
High resolution limit [Å]	1.050	1.050
Rmerge	0.093	0.506
Number of reflections	177114
<I/σ(I)>	57	3.4
Completeness [%]	99.8	99.5
Redundancy	5.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	Peg 8000, Na Cacodylate, Mg Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6	298	Peg 8000, Na Cacodylate, Mg Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6	298	Peg 8000, Na Cacodylate, Mg Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K