3LNU

Crystal structure of ParE subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	105.302, 105.302, 133.765
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.300 - 2.200
R-factor	0.236
Rwork	0.236
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.150	2.150
Number of reflections	43413
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	293	0.1M Na HEPES pH 7.6, 2% PEG 400, 1.8M ammonium sulfate, VAPOR DIFFUSION, temperature 293K