3LNR

Crystal structure of poly-HAMP domains from the P. aeruginosa soluble receptor Aer2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Detector technology	CCD
Collection date	2009-07-12
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97918, 0.97857, 0.97914, 0.95682
Spacegroup name	P 43 21 2
Unit cell lengths	113.612, 113.612, 66.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.640
Rwork	0.238
R-free	0.27100
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE (2.13)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.640	2.640
Number of reflections	12446
<I/σ(I)>	38.4	4.5
Completeness [%]	94.1	99.2
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	1.1 M Lithium sulfate, 15-18% glycerol, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K