3LNI
Crystal structure of E-cadherin EC12 E89A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.630, 77.122, 71.675 |
| Unit cell angles | 90.00, 115.25, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.1689 |
| Rwork | 0.166 |
| R-free | 0.22753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edh |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.093 |
| Number of reflections | 28177 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 99.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.25M ammonium sulfate, 0.1M MES pH 6.5, 26% PEG 5000 monomethylether, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
