3LND
Crystal structure of cadherin-6 EC12 W4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.979, 113.555, 67.907 |
| Unit cell angles | 90.00, 96.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.820 |
| R-factor | 0.211 |
| Rwork | 0.207 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.051 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.820 |
| Rmerge | 0.088 |
| Number of reflections | 20165 |
| <I/σ(I)> | 12.9 |
| Completeness [%] | 98.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 0.1M HEPES pH 7.5, 17% (v/v) isopropanol, 12% (w/v) PEG 4000, 9mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
