3LN9
Crystal structure of the fibril-specific B10 antibody fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 84.124, 84.124, 84.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.800 - 1.800 |
| R-factor | 0.19876 |
| Rwork | 0.197 |
| R-free | 0.23295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol0 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.918 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.624 |
| Number of reflections | 19838 | |
| <I/σ(I)> | 21.05 | 3.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 288 | 200mM Sodium Citrate, 2M Ammonium Sulphate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
