3LN8
The X-ray structure of Zf-RNase-1 from a new crystal form at pH 7.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-09 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.573, 49.183, 110.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.610 |
| R-factor | 0.146 |
| Rwork | 0.146 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ljd |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.054 | 0.115 |
| Number of reflections | 28093 | |
| <I/σ(I)> | 5 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 293 | 2.0 M ammonium sulphate, 0.1 M TRIS/acetate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
