3LMM
Crystal Structure of the DIP2311 protein from Corynebacterium diphtheriae, Northeast Structural Genomics Consortium Target CdR35
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 147.306, 102.030, 164.515 |
| Unit cell angles | 90.00, 116.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 3.000 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SHELX |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.525 |
| Number of reflections | 172016 | |
| <I/σ(I)> | 9.8 | 2.2 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M Na acetate (pH 4.6), 1M 1,6-hexanediol, and 10 mM CoCl2, Microbatch, under oil, temperature 291K |
