3LMH
Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.824, 110.047, 78.714 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.000 |
| R-factor | 0.21858 |
| Rwork | 0.216 |
| R-free | 0.25924 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lkm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (6.1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.099 | 0.481 |
| Number of reflections | 49217 | |
| <I/σ(I)> | 32.01 | 4.803 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 7.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 3350, 0.2M magnesium sulfate, 0.1M cacodylate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
