3LME
Structure of probable translation initiation inhibitor from (RPA2473) from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 112.185, 142.239, 147.365 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.740 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.563 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.119 | 0.072 | 0.905 |
| Number of reflections | 62233 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 12 | 11.4 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1M Acetate pH 4.5, 30% PEG 8K. 0.2M Lithium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
