3LMC
Crystal Structure of zinc-dependent peptidase from Methanocorpusculum labreanum (strain Z), Northeast Structural Genomics Consortium Target MuR16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97814 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.627, 60.889, 47.859 |
| Unit cell angles | 90.00, 101.19, 90.00 |
Refinement procedure
| Resolution | 29.373 - 1.997 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.264 |
| Number of reflections | 26475 | |
| <I/σ(I)> | 12.1 | 1.7 |
| Completeness [%] | 78.6 | |
| Redundancy | 2.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1m Sodium molybdate dehydrate, 0.1M Bis-Tris propane, 12% PEG 20000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
