3LMB
The crystal structure of the protein OLEI01261 with unknown function from Chlorobaculum tepidum TLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.090, 72.962, 79.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.830 - 2.100 |
| R-factor | 0.16924 |
| Rwork | 0.166 |
| R-free | 0.22193 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.004 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.830 | 2.154 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.416 |
| Number of reflections | 19329 | |
| <I/σ(I)> | 26.43 | 2.73 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 9.5 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Mg Formate, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
