3LMA
Crystal structure of the stage V sporulation protein AD (SpoVAD) from Bacillus licheniformis. Northeast Structural Genomics Consortium Target BiR6.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.957, 83.059, 103.662 |
| Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
| Resolution | 38.939 - 1.993 |
| R-factor | 0.1703 |
| Rwork | 0.168 |
| R-free | 0.21090 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.378 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.993 | 2.000 |
| Rmerge | 0.075 | 0.201 |
| Number of reflections | 142035 | |
| <I/σ(I)> | 20.1 | 4.53 |
| Completeness [%] | 97.4 | 84.2 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | icrobatch crystallization under paraffin oil | 7.5 | 291 | 40% PEG 1000, 0.1M ammonium phosphate, 0.1M HEPES, pH 7.5, icrobatch crystallization under paraffin oil, temperature 291K |
