3LM9
Crystal structure of fructokinase with ADP and Fructose bound in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2007-05-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 112.333, 112.333, 73.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.450 |
| R-factor | 0.15926 |
| Rwork | 0.157 |
| R-free | 0.19619 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3EPQ |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.876 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLEMAN2 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.110 | 0.710 |
| Number of reflections | 20173 | |
| <I/σ(I)> | 24 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 1.4 M Ammonium sulfate 0.1 M TRIS 10mM L-Fructose 20mM ADP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
