3LM0
Crystal Structure of human Serine/Threonine Kinase 17B (STK17B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-21 |
| Detector | MAR225 |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 83.540, 83.540, 115.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.610 - 2.350 |
| R-factor | 0.20566 |
| Rwork | 0.202 |
| R-free | 0.26749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3EHA.pdb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.545 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.610 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.131 | 0.750 |
| Number of reflections | 17724 | |
| <I/σ(I)> | 11.4 | 2.1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M (NH4)3(cit)20% PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
