3LLW
Crystal structure of geranyltransferase from helicobacter pylori 26695
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-01-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.040, 123.844, 111.617 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.19714 |
| Rwork | 0.196 |
| R-free | 0.25245 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.350 |
| High resolution limit [Å] | 2.299 | 2.290 |
| Number of reflections | 71235 | |
| <I/σ(I)> | 6.5 | 1.6 |
| Completeness [%] | 99.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1M HEPES PH 7.5, 1500MM LITHIUM SULFATE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
