3LLP
1.8 Angstrom human fascin 1 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97891 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.789, 72.659, 110.142 |
| Unit cell angles | 90.00, 130.28, 90.00 |
Refinement procedure
| Resolution | 29.450 - 1.800 |
| R-factor | 0.17452 |
| Rwork | 0.172 |
| R-free | 0.21959 |
| Structure solution method | SAD and MR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.601 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.373 |
| Number of reflections | 89183 | |
| <I/σ(I)> | 21.7 | 2.37 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM Hepes, pH 7.5, 20% PEG 4000, 2% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
