3LLO
Crystal structure of the STAS domain of motor protein prestin (anion transporter SLC26A5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.590, 61.590, 67.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.570 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.108 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.408 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.055 | 0.496 |
| Number of reflections | 20860 | |
| <I/σ(I)> | 8.5 | 3.9 |
| Completeness [%] | 99.3 | 97.6 |
| Redundancy | 11.3 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 1.8 M Ammonium sulfate, 4.5% (v/v) PEG 400, 0.1% (w/v) octyl-beta-D-glucopyranoside, 0.09 M MES, pH 6.5, VAPOR DIFFUSION, temperature 293.0K |
