3LLH

Crystal structure of the first dsRBD of TAR RNA-binding protein 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	90
Detector technology	CCD
Collection date	2009-12-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 31
Unit cell lengths	51.184, 51.184, 42.828
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.330 - 2.140
R-factor	0.185
Rwork	0.185
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rc7
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.140	2.140
Rmerge		0.807
Number of reflections	6917
<I/σ(I)>	12.175
Completeness [%]	100.0	100
Redundancy	5.8	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.44M SODIUM MALONATE pH7.0, 0.2M AMMONIUM SULFATE, 0.5% v/v ISOPROPANOL, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K