3LLA
Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.075, 109.808, 79.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.900 - 2.110 |
| R-factor | 0.21528 |
| Rwork | 0.213 |
| R-free | 0.26275 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lkm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.088 | 0.405 |
| Number of reflections | 42537 | |
| <I/σ(I)> | 37.69 | 5.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 9.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 18% PEG 8000, 0.2M sodium phosphate, 0.1M Tris-HCl , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
