3LL7
Crystal structure of putative methyltransferase PG_1098 from Porphyromonas gingivalis W83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.132, 78.485, 64.063 |
| Unit cell angles | 90.00, 104.65, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.18289 |
| Rwork | 0.181 |
| R-free | 0.20863 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.541 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.999 | 0.665 |
| Number of reflections | 47195 | |
| <I/σ(I)> | 26.3 | 2.36 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES, 10% PEG8000, 8% Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
