3LL1
Monomeric Griffithsin with a Single Gly-Ser Insertion and Mutations to Remove Residual Self-Association
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.400, 55.400, 61.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 0.970 |
| R-factor | 0.152 |
| Rwork | 0.152 |
| R-free | 0.16500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lky |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.304 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.082 | 0.780 |
| Number of reflections | 65427 | |
| <I/σ(I)> | 13.3 | 2 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 7.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | 0.1M citric acid, 3M NaCl, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
