3LL0
Monomeric Griffithsin with two Gly-Ser Insertions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-30 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 55.100, 55.100, 156.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lky |
RMSD bond length | 0.020 |
RMSD bond angle | 1.875 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.3) |
Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.090 | 0.690 |
Number of reflections | 16094 | |
<I/σ(I)> | 9.5 | 2.1 |
Completeness [%] | 97.9 | 93.5 |
Redundancy | 11.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M sodium acetate, 0.1 M CdCl, 30% w/v PEG 400, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |