3LKY
Monomeric Griffithsin with a Single Gly-Ser Insertion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 48.600, 48.600, 94.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.170 - 1.110 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| R-free | 0.15900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.967 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 2.980 | 1.100 |
| Rmerge | 0.063 | 0.057 | 0.583 |
| Number of reflections | 48948 | ||
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 97.9 | 99.5 | 99.7 |
| Redundancy | 6.2 | 12 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1 M sodium acetate, 0.15 M ammonium sulfate, 25% w/v PEG 3350, pH 4.0, vapor diffusion, hanging drop, temperature 298K |
