3LKV
Crystal structure of conserved domain protein from vibrio cholerae o1 biovar eltor str. n16961
Replaces: 2QH8Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2007-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97970, 0.97950, 1.28000 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 144.256, 144.256, 144.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.200 |
| R-factor | 0.17966 |
| Rwork | 0.178 |
| R-free | 0.20339 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.513 |
| Number of reflections | 26598 | |
| <I/σ(I)> | 10.3 | 10 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 30 | 30 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% PEG 550 MME 0.01M ZINC SULFATE 0.1M BIS-TRIS PH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
