3LKU
Crystal structure of S. cerevisiae Get4 in complex with an N-terminal fragment of Get5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97862 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.733, 108.733, 169.811 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.800 - 2.800 |
| R-factor | 0.18436 |
| Rwork | 0.182 |
| R-free | 0.22571 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0050) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.440 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.104 | 0.500 |
| Number of reflections | 29191 | |
| <I/σ(I)> | 20.3 | 4.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.1 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 17% PEG 6000, 0.1M Bis-Tris, 0.14M Ammonium sulfate, 0.01M L-proline, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
