3LKM

1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-18
Detector	ADSC QUANTUM 1
Wavelength(s)	0.918
Spacegroup name	P 21 21 2
Unit cell lengths	76.224, 83.777, 44.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.280 - 1.600
R-factor	0.19507
Rwork	0.194
R-free	0.22364
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.135
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.066	0.190
Number of reflections	37007
<I/σ(I)>	46.52	9.77
Completeness [%]	96.6	78.7
Redundancy	10.3	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	20% PEG 3350, 0.2M potassium phosphate, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	20% PEG 3350, 0.2M potassium phosphate, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K