3LKD
Crystal Structure of the type I restriction-modification system methyltransferase subunit from Streptococcus thermophilus, Northeast Structural Genomics Consortium Target SuR80
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.866, 83.128, 96.678 |
Unit cell angles | 90.00, 98.13, 90.00 |
Refinement procedure
Resolution | 48.790 - 2.250 |
Rwork | 0.186 |
R-free | 0.21000 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AutoSol |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.083 | 0.279 |
Number of reflections | 88228 | |
<I/σ(I)> | 18.5 | 4.9 |
Completeness [%] | 99.1 | 98.7 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 3350, 0.2M ammonium tartrate, 4% N-propanol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |