3LJX

Crystal Structure of MmoQ Response regulator (fragment 20-298) from Methylococcus capsulatus str. Bath, Northeast Structural Genomics Consortium Target McR175G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.97853
Spacegroup name	P 31 2 1
Unit cell lengths	91.489, 91.489, 83.075
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.947 - 2.300
R-factor	0.1903
Rwork	0.189
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3LJV
RMSD bond length	0.008
RMSD bond angle	1.065
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.067	0.329
Number of reflections	33201
<I/σ(I)>	16.8	4.1
Completeness [%]	95.9	97.6
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution:, VAPOR DIFFUSION, HANGING DROP