3LJS
Crystal structure of Fructokinase from Xylella fastidiosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.019, 93.625, 179.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.460 - 1.970 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 83152 | |
| <I/σ(I)> | 7.1 | 6.4 |
| Completeness [%] | 99.3 | 49.1 |
| Redundancy | 14.1 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 30mM Potassium Phosphate Dihydrogen 15% PEG 8K , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
