3LJK
Glucose-6-phosphate isomerase from Francisella tularensis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 115.064, 115.064, 84.455 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.300 - 1.480 |
| R-factor | 0.126 |
| Rwork | 0.124 |
| R-free | 0.15800 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.629 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.093 | 0.679 |
| Number of reflections | 106870 | |
| <I/σ(I)> | 12.7 | 2.08 |
| Completeness [%] | 99.1 | 92 |
| Redundancy | 9.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.2 M Ca(OAc)2, 0.1 M MES buffer, 10% 2-propanol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
