3LIS
Crystal Structure of the Restriction-Modification Controller Protein C.Csp231I (Monoclinic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.010, 29.530, 64.380 |
| Unit cell angles | 90.00, 101.91, 90.00 |
Refinement procedure
| Resolution | 31.500 - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.061 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.276 | |
| Number of reflections | 12439 | |
| <I/σ(I)> | 11.7 | 4.1 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M malate-MES-Tris (MMT), 20% PEG 1500, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
