3LIJ
Crystal structure of full length CpCDPK3 (cgd5_820) in complex with Ca2+ and AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-18 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.080, 88.060, 58.610 |
| Unit cell angles | 90.00, 118.07, 90.00 |
Refinement procedure
| Resolution | 19.560 - 1.900 |
| Rwork | 0.203 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 40219 | |
| <I/σ(I)> | 12.45 | 2.63 |
| Completeness [%] | 99.1 | 94.4 |
| Redundancy | 7.39 | 6.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18% PEG3350, 0.1M Ammonium Tartrate, 5 mM CaCl2, 5 mM AMPPNP, 1 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
