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3LIE

Crystal Structure of the extracellular domain of the putative histidine kinase vpHK1S-Z8

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X4A
Synchrotron siteNSLS
BeamlineX4A
Temperature [K]100
Detector technologyCCD
Wavelength(s)0.97921
Spacegroup nameP 21 21 2
Unit cell lengths78.049, 114.953, 69.624
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution39.028 - 2.590
R-factor0.258
Rwork0.254
R-free0.32600
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.017
RMSD bond angle1.708
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER (1.3.1)
Refinement softwareREFMAC (5.5.0066)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0002.690
High resolution limit [Å]2.6005.6002.600
Rmerge0.0770.0720.355
Number of reflections19533
<I/σ(I)>14.2
Completeness [%]97.797.195.9
Redundancy4.24.84.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION72932M NaCl, 0.2M MgCl, 0.1M Tris, pH 7.0, vapor diffusion, temperature 293K

229380

PDB entries from 2024-12-25

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