3LI8
Crystal Structure of the extracellular domain of the putative histidine kinase mmHK1S-Z2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-02 |
| Wavelength(s) | 0.96784 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.456, 87.693, 99.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.450 - 1.750 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.094 | 0.087 | 0.301 |
| Number of reflections | 30021 | ||
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 100.0 | 99.9 | 99.8 |
| Redundancy | 7.3 | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 15% PEG3350, 0.21M NH4SO4, 0.1M sodium cacodylate, pH 5.6, vapor diffusion, temperature 293K |
