3LI6
Crystal structure and trimer-monomer transition of N-terminal domain of EhCaBP1 from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 3 |
| Unit cell lengths | 89.583, 89.583, 35.049 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.490 - 2.502 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nxq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.121 |
| Data reduction software | AUTOMAR |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Total number of observations | 39681 | |
| Number of reflections | 10746 | |
| <I/σ(I)> | 10.3 | 10.3 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 289 | 18% PEG 400, 0.05M NaOAc, 5mM CaCl2, 10% Isopropanol, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
