3LHS
Open Conformation of HtsA Complexed with Staphyloferrin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.564, 43.515, 75.324 |
| Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
| Resolution | 29.900 - 1.300 |
| R-factor | 0.15528 |
| Rwork | 0.154 |
| R-free | 0.18571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.056 | 0.355 |
| Number of reflections | 69549 | |
| <I/σ(I)> | 12 | 3.1 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 298 | 0.1 M Hepes, 24% Jeffamine ED-2001, pH 7.0, hanging drop, temperature 298K |
