3LHR

Crystal structure of the SCAN domain from Human ZNF24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-24
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	43.055, 50.029, 91.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.730 - 1.900
R-factor	0.184
Rwork	0.182
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.024
RMSD bond angle	1.861
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.082	0.456
Number of reflections	15875
<I/σ(I)>	12.3
Completeness [%]	97.3	76.5
Redundancy	13.3	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Protein solution at 1.5 mM concentration was mixed 1:1 with a reservoir solution composed of 100 mM HEPES buffer, pH 7.5, 40 mM MgCl2, 24% MEPEG 2000, 2 mM thimerosal. Crystals were cryoprotected with reservoir solution supplemented with 10% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 291K