3LHI
Crystal structure of Putative 6-phosphogluconolactonase(YP_207848.1) from Neisseria gonorrhoeae FA 1090 at 1.33 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97951 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 63.237, 63.237, 106.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.382 - 1.330 |
R-factor | 0.133 |
Rwork | 0.132 |
R-free | 0.16100 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.702 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.382 | 27.382 | 1.380 |
High resolution limit [Å] | 1.330 | 2.860 | 1.330 |
Rmerge | 0.036 | 0.023 | 0.594 |
Number of reflections | 52593 | 10997 | 6413 |
<I/σ(I)> | 17.47 | 47.6 | 1.4 |
Completeness [%] | 90.9 | 99.3 | 55.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 0.2000M NH4F, 20.0000% PEG-3350, No Buffer pH 6.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |