3LHE
The crystal structure of the C-terminal domain of a GntR family transcriptional regulator from Bacillus anthracis str. Sterne
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 39.595, 76.003, 107.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.100 - 1.620 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.22830 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.100 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.074 | |
| Number of reflections | 20951 | |
| <I/σ(I)> | 32.1 | 1.6 |
| Completeness [%] | 98.9 | 91.6 |
| Redundancy | 5.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.05 M Bis-Tris, 45% v/v PPGP 400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
