3LH8
Crystal structure of mouse VPS26B in spacegroup P41 21 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-27 |
| Wavelength(s) | 0.95364 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.157, 118.157, 193.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.900 - 2.600 |
| R-factor | 0.2627 |
| Rwork | 0.261 |
| R-free | 0.29210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r51 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.295 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.900 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.065 | 0.369 |
| Number of reflections | 79550 | |
| <I/σ(I)> | 21.6 | 3.3 |
| Completeness [%] | 98.9 | 93.2 |
| Redundancy | 6.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 2M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
