3LH4
Crystal Structure of Sialostatin L2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97902 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 95.451, 95.451, 95.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.480 - 1.800 |
| R-factor | 0.17391 |
| Rwork | 0.173 |
| R-free | 0.19602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.480 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.095 | 0.195 |
| Number of reflections | 13472 | |
| <I/σ(I)> | 22.1 | 26.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 29.9 | 28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 1.8 M Ammonium sulfate, 0.1 M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
