3LGJ
Crystal structure of single-stranded binding protein (ssb) from Bartonella henselae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.530, 93.530, 115.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.700 - 2.500 |
R-factor | 0.228 |
Rwork | 0.225 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kaw |
RMSD bond length | 0.014 |
RMSD bond angle | 1.510 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.700 | 2.570 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.044 | 0.595 |
Number of reflections | 10787 | 788 |
<I/σ(I)> | 34.12 | 4.9 |
Completeness [%] | 99.1 | 99.7 |
Redundancy | 13.2 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | JCSG+ condition G11, 0.1 M BisTris, 2 M ammonium sulfate, crystal tracking ID 202927g11, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |