3LGJ
Crystal structure of single-stranded binding protein (ssb) from Bartonella henselae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.530, 93.530, 115.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.700 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kaw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.044 | 0.595 |
| Number of reflections | 10787 | 788 |
| <I/σ(I)> | 34.12 | 4.9 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 13.2 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | JCSG+ condition G11, 0.1 M BisTris, 2 M ammonium sulfate, crystal tracking ID 202927g11, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
