3LGJ

Crystal structure of single-stranded binding protein (ssb) from Bartonella henselae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-12
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	93.530, 93.530, 115.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.700 - 2.500
R-factor	0.228
Rwork	0.225
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kaw
RMSD bond length	0.014
RMSD bond angle	1.510
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.700	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.044	0.595
Number of reflections	10787	788
<I/σ(I)>	34.12	4.9
Completeness [%]	99.1	99.7
Redundancy	13.2	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	JCSG+ condition G11, 0.1 M BisTris, 2 M ammonium sulfate, crystal tracking ID 202927g11, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K