3LGA
Crystal structure of P. abyssi tRNA m1A58 methyltransferase in complex with S-adenosyl-L-homocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 31 |
| Unit cell lengths | 138.216, 138.216, 121.119 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.860 - 2.050 |
| R-factor | 0.19959 |
| Rwork | 0.199 |
| R-free | 0.21768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lhd |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.824 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.860 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.091 | 0.706 |
| Number of reflections | 162057 | |
| <I/σ(I)> | 11.5 | 2.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2.4M ammonium sulfate, 0.2M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
