3LG3
1.4A Crystal Structure of Isocitrate Lyase from Yersinia pestis CO92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.163, 115.133, 174.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.558 - 1.400 |
| R-factor | 0.1329 |
| Rwork | 0.131 |
| R-free | 0.16530 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.913 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CRANK |
| Refinement software | PHENIX ((phenix.refine: 1.4_115)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.079 | 0.519 |
| Number of reflections | 185843 | |
| <I/σ(I)> | 28.3 | 2.86 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M LiCitrate, PEG3350 20%, 10mm iso Citric acid, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
