3LFV
crystal structure of unliganded PDE5A GAF domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9796, 0.94, 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 144.189, 144.189, 134.867 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.24267 |
| Rwork | 0.240 |
| R-free | 0.28456 |
| Structure solution method | MAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.667 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.840 |
| Number of reflections | 40247 | |
| <I/σ(I)> | 19.5 | 4.7 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 21.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | The unliganded PDE5A (20 mg/mL) was crystallized by vapor diffusion against the well buffer of 10% PEG3350, 5% MPD, 0.1 M sodium citrate, pH5.6, 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
