3LFP
Crystal Structure of the Restriction-Modification Controller Protein C.Csp231I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 137.370, 137.370, 137.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.240 - 2.000 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y7y |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.753 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.408 |
| Number of reflections | 7753 | 1052 |
| <I/σ(I)> | 42.78 | 11.3 |
| Completeness [%] | 97.2 | 94.2 |
| Redundancy | 38.7 | 38.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M Na HEPES, 1.4M Tri-sodium citrate dihydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
